The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. 2 only: SpectraMax Mini Update. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. Three low volume 24-well bottom slides. 1. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Minimize manual manipulation of samples and data processing, and improve laboratory efficiency. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. SpectraDrop High-Throughput Screening (HTS) Kit. . The dual monochromator optics allow. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance values to a 1-cm pathlength so that they are the same as values obtained in a 1-cm cuvette. Using the optimized wavelength settings, the SPECTRAmax GEMINI XS fluorescence microplate reader can distinguish between live and dead cells using this assay. A preconfigured protocol is available in the software to. SpectraMax L Microplate ReaderThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. No additional manuals are available. Add to Cart. The SpectraMax microplate readers can be used to accurately quantify ROS levels using a luminescence-based assay. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. With optimized reagents, option to operate the. The drawing shows a standard microplate of 15 mm height covered with a lid. microplate reader. Contact our support team with your questions regarding Molecular Devices products and applications. Examples: “Press carefully to engage,” “ Do not. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. A. Keywords: spectramax mini multi-mode microplate reader, spectramax mini, spectramax mini microplate reader Created Date: 5/25/2022 9:57:03 AMFully Warranty. com. Multimode Microplate Reader. The Gemini XPS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. San Jose, Calif. 306 plate air cal fail,low light. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. . SpectraMax ® M2 Multi-Mode Microplate Reader. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. begin reading microplates with seven mouse clicks. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Microplate Spectrophotometers. 1. 4 μL DNA standards. . The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Open the top cover of the instrument. Path Check Pathlength Measurement every Gemini EM Reader. 314 low lamp level warning. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. Use the provided 3. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. Use the provided 3. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 20 μL of Component A was added to each well of the microplate. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Reporter Assay. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. We would like to show you a description here but the site won’t allow us. ¨0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingThe SpectraMax iD5 Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Temperature control and shaking come as standard. Additionally, user-installable options allow the SpectraMax i3 System to. The guide also covers the software features, the specifications, and the accessories of the systems. 997 (n = 3). The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. A microplate reader as The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Sensitive fluorescent quantitation of DNA with the Quant-iT. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. We would like to show you a description here but the site won’t allow us. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. 306 plate air cal. Detection modalities include absorbance (UV-Vis Abs) and. 1) Select the correct plate and the wells that you want to measure. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. Products : General, Microplate Readers. 0 out of 5 $ 11,999. Self Service Available. Molecular Devices SpectraMax M2 Multimode Plate Reader. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Total volume (except deep. flexibility of monochromator-based systems. with SpectraMax Microplate Readers Introduction Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. e. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. 0 out of 5 $ 5,999. Other features and benefits include: Eliminate changing out filters. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. In addition, the SpectraMax iD5Next generation SpectraMax® absorbance microplate readers. The powerful analysis tools in the software generate a standard curve and calculated data. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. These robust readers have been placed in labs from. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. . A: Microplate Preparation. Transcreener ® ADP. Download PDF. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. 0 out of 5 $ 9,995 $ 9,495. Win7 Manual Spectramax-L or StakMax Driver Installation after Initial Install. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The plate reader optics are tunable for absorbance. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. For readers without a cuvette port, simply check the box next to ‘PathCheck’. 58% for the detection module and 2. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. HTRF-compatible SpectraMax Multi-Mode Readers. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Highly customizable low volume. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. 2. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The Quant-iT PicoGreen dsDNA Assay Kit, when run on a SpectraMax microplate reader with SoftMax Pro Software, is a quick, sensitive detection method for double-stranded DNA. The plate reader can measure multiple readouts, including UV and visible absorbance. . Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. . Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. Blank subtraction and standard curve fitting were done automatically by the software. In addition, the SpectraMax® iD5 can be expanded to include bottom. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Find regional support. ELISA Plate Readers / Microplate Readers. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. 030 fmol Fluorescence Polarization (FP). See SpectraMax® Microplate Reader User Manual Download Page. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Molecular Devices. Here, the CVs for each plate were less than 3, and the difference between mean. Figure 1. Molecular Devices SpectraMax M2E Microplate Reader. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. 1. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). For the calculation of the ratiometric biosensor signal, the emission intensity at 510 nm was recorded upon an excitation at 380 nm and 470 nm and the former was divided by the latter (Exc. It measures the optical density (OD) of samples in both 96- and 384-well. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. FI assay. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The calculated LOQ values were 0. In this product video, see how the SpectraMax M5 quickly reads samples. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 0 out of 5 $ 2,999-20%. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. both cuvette-based and microplate reader applications Microplate Readers. 33 Dramatic aggregation of Alzheimer A b by Cu(II) is induced by conditions representing physiological acidosis . Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. All . The 2-channel configuration is available with 0, 2, or 4 injectors. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. Molecular Devices Spectramax iD3 Microplate Reader. Thank you!. Choose from a three- (M3), four- (M4), orIn this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. on the SpectraMax iD3 microplate reader Introduction The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path length. 7°C (gradient heating design). The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Table of Contents. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Guides: SpectraMax Mini Installation Guide. limit of detection (LLD) for ATP was estimated for each microplate reader using the . The data suggests that there is little interference from dead cells when live cells are analyzed, however, there may be some interference from live cells when dead cells are analyzed. SpectraMax L Microplate Reader This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. ATP standard curves read on SpectraMax microplate readers. When the user applies a set temperature of 37°C, the top is heated to 37. Avoid losing high signal. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein. Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Figure 1. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. To resume collecting kinetic measurements within the same file, press the Append button. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 µL sample volume in a. microplate reader. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow toFlexStation® 3 Multi-Mode Microplate Reader. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Fully tested with validations, warranty. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Italic and boldface type are used for emphasis. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. SpectraMax Spectrofluorometers. We would like to show you a description here but the site won’t allow us. This section contains the following topics:滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. Introduction. SpectraMax Spectrofluorometers. 2. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Example: [Drawer]. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. 1 software Download page. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. 40266 Email: drugdiscoverytech@lifetech. 0 out of 5 $ 5,999. Italic and boldface type are used for emphasis. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. 0200-6263. For more sample throughput, both readersSupplier: Molecular Devices. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast. 3) This warranty. These lab workhorses are used in drug discovery, research, bioassay. The 2-channel configuration is available with 0, 2, or 4 injectors. Please refer to the Obsolete Microplate Reader Models for more information. : 2 Oct 12 Page 6 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Read More. Molecular Devices: Molecular Devices. AlphaScreen. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell. This unlocks the transport slide. microplate reader. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today launched its. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Microplate Readers. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. Supplier: Molecular Devices. The SpectraMax M2 and M2e are versatile and reliable. Results are shown in Table 1. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Step 1: Open the SoftMax Pro IMAP FP protocol. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Microplate automation and detection instruments for walk away operation and high quality results. Category 2 and 3 microplate readers use gradient heating as well. Multimode Microplate Readers. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Step 2: Launch SoftMax Pro Software. View the Molecular Devices website. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalSpectraMax Microplate Readers. configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking andThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expandthe boundaries of your research capabilities. Data exemplifying linear ranges from the three different kits are presented in Figure 2. no. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 76358-624. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. 3) At the bottom of the strip, click on "Show. 0 out of 5 $ 5,999. com 4. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Materials. SpectraMax Mini User Guide. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. In addition, the SpectraMax® iD5 can be expanded to include bottom. Or scan up to 384 wells at any time to determine the best assay conditions. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. Lysozyme contains two tryptophan residues that. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. Figure 1. The SpectraMax® iD3 Multi Mode Microplate . 2 software: see SoftMax® Pro 7. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. #6590-0131) Fluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. 1. Table 1. For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. Examples: “Press carefully to engage,” “ Do not. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Fluorescence analysis of the colonies from the dilution series was performed in a microplate reader (SpectraMax iD3). Microplate Readers. Users can install the ScanLater Western Blot Detection Cartridge in the SpectraMax Paradigm SystemMicroplate readers read biological, chemical or physical events that occur on a microtiter plate. Unparalleled performance on a personalized platform. In . The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Download PDF. 305 reference level saturation. For a higher . IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. 303 unable to cal dark current. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. The SpectraMax® iD3 multi-mode microplate reader measures absorbance, fluorescence and luminescence and is ideal for busy multi-user, multi-application labs. Microplate readers’ standard detection modes. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. flat meniscus), but is approx 15 mL. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Figure 3. limit of detection (LLD) for ATP was estimated for each microplate reader using the . SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMinimum volume and maximum throughput with 96- or 384-well microplate compatibility. Published Dec 16, 2020 | Updated Dec 16, 2020. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Measure more accurately. . The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. It can run samples based on pre-defined. The SpectraMax 190 reader can be integrated with Molecular . The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. The screw remains inside the instrument. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. 100 readings per well gives optimal Z’ values for this assay. The plate reader optics are tunable for. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Summary of Contents for Molecular Devices SpectraMax M2. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. In addition, the SpectraMax iD5 can be expanded to. Cell Cytotoxicity assay procedure. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. 5 87.